Chapter 23
Biotechnology
Encircle the most appropriate answer among the following options
1. Mendel’s work was rediscovered in choose which one is correct:
- 1868
- 1882
- 1900
- 1950
2. The work of biotechnology is choose which one is correct:
- Insulin
- Drugs
- Pollution
- All of the above
3. Production of identical copying of gene is choose which one is correct:
- Replication
- Cloning
- Reproduction
- Multiplication
4. Which of the following techniques produces large number of copies of genes?
- PCR
- Recombinant DNA technology
- RFLP
- None of the above
5. The technique used to produce copies of genes in laboratory is choose which one is correct:
- PCR
- Recombinant DNA technology
- RFLOP
- None of the above
6. Which of the followings is used to transfer genes?
- Molecular scissors
- Molecular vector
- Expression system
- None of the above
7. The system used for the formation of specific product is choose which one is correct:
- Molecular scissors
- Molecular vector
- Expression system
- Gene of interest
8. Genes are cut by choose which one is correct:
- Gene of interest
- Expression system
- Molecular vector
- Endonucleases
9. The genes which are synthesized from mRNA by reverse transcriptase are
called choose which one is correct:
- Endonucleases
- Plasmids
- Coda
- None of the above
10. Restriction enzymes are present in choose which one is correct:
- Virus
- Bacteria
- Plasmids
- All of the above
11. The first restriction enzyme was isolated by choose which one is correct:
- Kary Mullis
- Hamiton
- Sanger
- Maxam
12. Palindromic sequences are present in choose which one is correct:
- Single phase
- Repeated form
- Reverse order
- Similar order
13. Number of restriction enzymes discovered so far are choose which one is correct:
- 200
- 300
- 400
- 500
14. The number of frequently used restriction enzymes are choose which one is correct:
- 10
- 20
- 30
- 40
15. The commonly used restriction enzyme is choose which one is correct:
- E.Coli
- EcoR11
- EcoR1
- EcoR111
16. Natural extra chromosomal circular DNA are choose which one is correct:
- Endonucleases
- Restriction enzyme
- Plasmids
- Phage virus
17. Which of the followings is not a vector?
- pSc 101
- pBR-322
- EcoR1
- Phage virus
18. The plasmids having resistant gene against tetracycline and ampicillin are choose which one is correct:
- pSc 101
- pBR-322
- EcoR1
- Phage virus
19. Which of the following phages is use as vector?
- Bacteriophage
- Lambda phage
- T phage
- All of the above
20. Pieces of DNA are jointed by choose which one is correct:
- Chimaeric DNA
- DA ligase
- Plasmids
- Endonucleases
21. Match recombinant DNA with one of the followings choose which one is correct:
- Chimaeric DNA
- DNA ligase
- Plasmids
- Endonucleases
22. Recombinant DNA are expressed in choose which one is correct:
- Man
- Virus
- Bacteria
- None of the above
23. The chemical that makes the bacterial membrane permeable is choose which one is correct:
- NaCl
- CaCl2
- Na2CO3
- CaCO3
24. The single standard nucleotide sequence that hybridize into certain piece is choose which one is correct:
- Clone
- Probe
- REFLP
- Endonuclease
25. Which of the followings form genomic library?
- Bacteria
- Bacteriophage
- Segment of DNA
- None of the above
26. PCR technique was developed by choose which one is correct:
- Kary Mullis
- Hamilton
- Sanger
- Maxam
27. Which of the followings is irrelevant for PCR?
- Primer DNA
- Recombinant DNA
- DNA polymerase
- Thermocycler
28. Match target DNA with one of the followings choose which one is correct:
- Primer DNA
- Recombinant DNA
- DNA polymerase
- Thermocycler
29. The enzyme extracted from Thermus aquaticus is choose which one is correct:
- Primer DNA
- Recombinant DNA
- DNA polymerase
- Thermocycler
30. The steps involved in DNA finger printing choose which one is correct:
- RFLP
- Probes
- PCR
- All of the above
31. The collection of different sized fragments is choose which one is correct:
- Probes
- RFLP
- PCR
- Gel
32. DNA analyzer is used for choose which one is correct:
- Forensic
- Diagnosis
- Evolution
- All of the above
33. The use of DNA analysis for convicting the criminal of rape is used in choose which one is correct:
- Diagnosis
- Forensic
- Evolution
- All of the above
34. Match Sanger’s method for generation of pieces of DNA with one of the
followings choose which one is correct:
- Deoxyribonucleotide
- Ribonucleoside
- Dideoxyribonucleoside
- DNA threads
35. Match Maxam Gilbert method for generation of pieces of DNA with one of
the followings choose which one is correct:
- Deoxyribonucleotide
- Dexoyribonucleoside
- Dideoxyribonucleoside
- DMA threads
36. Pieces of DNA are separated on choose which one is correct:
- Agarol
- Agarose gel
- Agar
- All of the above
37. In gene sequencing the nucleotide strained with fluorescent dye are choose which one is correct:
- Initiation nucleotides
- Middle nucleotides
- Terminating nucleotides
- None of the above
38. In gene sequencing, the colour of the fluorescent bands are read by choose which one is correct:
- Computer
- Gel
- Detector
- Laser beam
39. The nucleotide sequence is stored by choose which one is correct:
- Computer
- Gel
- Detector
- Laser beam
40. The DNA sequence of which of the followings have been done?
- Chloroplast
- Mitochondria
- Yeast
- All of the above
41. Which of the followings is model plant whose gene sequencing has been completed?
- Yeast
- Pea
- Arabidopsis
- Plum
42. The gene sequence of which of the following chromosomes was completed in
1999 choose which one is correct:
- 12
- 18
- 21
- 22
43. The smallest human chromosomes is choose which one is correct:
- 10
- 22
- 23
- Y
44. Which of the followings helped scientist to pinpoint disease causing genes?
- PCR
- Recombinant DNA
- RFLP
- Probe
45. The number of base sequences in man is choose which one is correct:
- 2 billion
- 3 billion
- 4 billion
- 5 billion
46. The human genomic project will take how many volume of encyclopedia choose which one is correct:
- 100
- 200
- 300
- 400
47. Each piece of DNA should be choose which one is correct:
- 500 nucleotide
- 1000 nucleotide
- 3000 nucleotide
- 4000 nucleotide
48. The copy of DNA pieces in human genomic project is made by choose which one is correct:
- PCR
- Recombinant DNA
- RFLP
- Probe
49. The entire genome has been sequence by the company of choose which one is correct:
- J. Craig Venter
- Hamilton
- Sanger
- Maxam
50. Organisms with foreign genes are called choose which one is correct:
- Recombinant
- Hybrids
- Transgenic
- Modified
51. Transgenic bacteria are produced in large vats called choose which one is correct:
- Thermocycler
- Bioreactors
- Reactors
- Electrophoresis
52. Which of the following products is prepared by recombinant DNA technology?
- Hepatitis B vaccine
- Growth hormone
- Insulin
- All of the above
53. The degradation of pollutants with the help of recombinant bacteria is called choose which one is correct:
- Biofilters
- Biodegradation
- Bioabsorption
- None of the above
54. The bacteria used in industries for control of pollution are choose which one is correct:
- Bioabsorption
- Biodegradation
- Biofilters
- None of the above
55. The genes which cause self destruction of bacteria are choose which one is correct:
- Phage genes
- Suicide genes
- Recombinant genes
- Plasmid genes
56. Naturasweet is choose which one is correct:
- Sugar
- Starch
- Aspartame
- Glucose
57. The bacteria are used in mining industry for choose which one is correct:
- Bioabsorption
- Biodegradation
- Biofilters
- Bioleaching
58. The plant cells whose cell wall is removed are choose which one is correct:
- Naked cells
- Cullous
- Protoplast
- All of the above
59. In which of the followings plant pest and herb resistant genes are not used?
- Cotton
- Corm
- Yeast
- Potato
60. The acreage of transgenic plants in 1999 was choose which one is correct:
- 50 million
- 70 million
- 90 million
- 100 million
61. Green revolution was launched in choose which one is correct:
- 1960
- 1970
- 1990
- 2000
62. Scientists are working on which of followings for increasing yield choose which one is correct:
- Stomata
- RuBP carboxylase
- C4 cycle
- All of the above
63. Biodegradable plastic is obtained from choose which one is correct:
- Yeast
- Mouse eared cress
- Bacteria
- Bioreactos
64. The antibody which can deliver radio isotopes to tumor cells is obtained
from choose which one is correct:
- Yeast
- Weed
- Corn
- Wheat
65. The antibody which is used for the treatment of genital herps is obtained
from choose which one is correct:
- Yeast
- Soya bean
- Corn
- Wheat
66. Gene pharming is used for obtaining choose which one is correct:
- Milk
- Meat
- Antibodies
- Drugs
67. In gene pharming egg is fertilized choose which one is correct:
- In vitro
- In vivo
- In bioreactor
- None of the above
68. Antithrombin is produced in choose which one is correct:
- Sheep
- Goat
- Mice
- Bovine
69. The organisms which are used to produce human growth hormone in their
urine are choose which one is correct:
- Sheep
- Goat
- Mice
- Bovine
70. The production of genetically identical copies of the organisms by asexual
reproduction is called choose which one is correct:
- Recombinant DNA
- Cloning
- Gene pharming
- None of the above
71. The first animal cloned was choose which one is correct:
- Sheep
- Dolly
- Mice
- Bovine
72. In the cloning of mice the second nucleus is obtained from choose which one is correct:
- Uterus cells
- Cumulus cells
- Skin cells
- None of the above
73. The insertion of genetic material into human cells for the treatment of a
disorder is called
- Gene cloning
- Gene pharming
- Gene therapy
- Gene sequencing
74. The disease not treated by Ex vivo gene therapy is choose which one is correct:
- SCID
- Diabetes
- Hypercholesterolemia
- All of the above
75. The enzyme ADA is involved in maturation of choose which one is correct:
- RBC
- Platelets
- Lymphocyte
- Macrophages
76. The organism used as vector during treatment of SCID by gene therapy is choose which one is correct:
- Plasmids
- Phage
- Retroviruses
- None of the above
77. The genes for the treatment of SCID are introduced in choose which one is correct:
- Bone marrow
- Heart
- Liver
- None of the above
78. The hypercholesterolemia causes at young age choose which one is correct:
- Diabetes
- Blood pressure
- Heart attack
- All of the above
79. The genes for the treatment of hypercholesterolemia are introduced in choose which one is correct:
- Bone marrow
- Heart
- Liver
- None of the above
80. In which of these in vivo gene therapy is used?
- Cystic fibrosis
- Cancer
- Coronary angioplasty
- All of the above
81. In cystic fibrosis, the gene codes for trans membrane carrier of which ion is
missing choose which one is correct:
- Na
- K
- Cl
- Ca
82. The microscopic vesicles formed during gene therapy of cystic fibrosis are
called choose which one is correct:
- Ribosomes
- Lipososmes
- Endosomes
- Microsomes
83. The solution of Liposomes is sprayed on choose which one is correct:
- Skin
- Joints
- Nostrils
- Mouth
84. The gene therapy in cancer makes the patient tolerant for choose which one is correct:
- Radiotherapy
- Chemotherapy
- Endoscopies
- Gene therapy
85. These are used in angiosplasty choose which one is correct:
- Balloon
- Plasmids
- Gene therapy
- All of the above
86. Which of the following diseases is not treated by in vivo gene therapy?
- Haemophilia
- Diabetes
- Parkinson’s diseases
- All of the above
87. The plants cells can develop complete plants. So they are called choose which one is correct:
- Callous
- Totipotent
- Clone
- None of the above
88. Complete carrot plant was developed from tiny part of phloem by choose which one is correct:
- J. Craig Venter
- Hamilton
- Sanger
- F.C. Steward
89. F. C Steward provided the phloem cells choose which one is correct:
- Sugars
- Vitamins
- Coconut milk
- All of the above
90. An undifferentiated group of cells is called choose which one is correct:
- Callous
- Totipotent
- Clone
- None of the above
91. Callous can form choose which one is correct:
- Root
- Stem
- Shoot
- Complete plant
92. In micropropagation, the structure used is choose which one is correct:
- Stem
- Meristem
- Callous
- Totipotent
93. In micropropagation of meristem, the hormone used is choose which one is correct:
- Gibberellins
- Auxins
- Abscisic acid
- All of the above
94. The plants produced by micropropagation of meristem are called choose which one is correct:
- Callous
- Seedling
- Clonal
- Somaclonal
95. Which of the following is virus free?
- Callous
- Seedling
- Clonal plant
- Adult plant
96. The naked cells of plant are called choose which one is correct:
- Protoplasm
- Cytoplasm
- Protoplast
- Callous
97. The protoplast regenerates and produces choose which one is correct:
- Callous
- Seedling
- Clonal
- Clump
98. The clumps produce choose which one is correct:
- Callous
- Somatic embryo
- Clonal plant
- Adult plant
99. Somatic embryo are encapsulated in gel and are called choose which one is correct:
- Seeds
- Artificial seed
- Clonal plant
- Clump
100. Millions of artificial seed are produced in large tanks called choose which one is correct:
- Thermocycler
- Biopole
- Bioreactors
- None of the above
101. The artificial seeds are produced of which of the following plants?
- Corn
- Tomato
- Mango
- Roses
102. The plants produced by micropropagation from single cell are called choose which one is correct:
- Callous
- Seedling
- Clonal
- Somaclonal
103. Anther is cultured in a medium containing choose which one is correct:
- Sugars
- Vitamins
- Coconut milk
- All of the above