12th Class Biology Mcqs with Answers Chapter 23 | 2nd year Biology Mcqs With Answers

Chapter 23

Biotechnology

Encircle the most appropriate answer among the following options

1. Mendel’s work was rediscovered in choose which one is correct:

• 1868
• 1882
• 1900
• 1950

2. The work of biotechnology is choose which one is correct:

• Insulin
• Drugs
• Pollution
• All of the above

3. Production of identical copying of gene is choose which one is correct:

• Replication
• Cloning
• Reproduction
• Multiplication

4. Which of the following techniques produces large number of copies of genes?

• PCR
• Recombinant DNA technology
• RFLP
• None of the above

5. The technique used to produce copies of genes in laboratory is choose which one is correct:

• PCR
• Recombinant DNA technology
• RFLOP
• None of the above

6. Which of the followings is used to transfer genes?

• Molecular scissors
• Molecular vector
• Expression system
• None of the above

7. The system used for the formation of specific product is choose which one is correct:

• Molecular scissors
• Molecular vector
• Expression system
• Gene of interest

8. Genes are cut by choose which one is correct:

• Gene of interest
• Expression system
• Molecular vector
• Endonucleases

9. The genes which are synthesized from mRNA by reverse transcriptase are

called choose which one is correct:

• Endonucleases
• Plasmids
• Coda
• None of the above

10. Restriction enzymes are present in choose which one is correct:

• Virus
• Bacteria
• Plasmids
• All of the above

11. The first restriction enzyme was isolated by choose which one is correct:

• Kary Mullis
• Hamiton
• Sanger
• Maxam

12. Palindromic sequences are present in choose which one is correct:

• Single phase
• Repeated form
• Reverse order
• Similar order

13. Number of restriction enzymes discovered so far are choose which one is correct:

• 200
• 300
• 400
• 500

14. The number of frequently used restriction enzymes are choose which one is correct:

• 10
• 20
• 30
• 40

15. The commonly used restriction enzyme is choose which one is correct:

• E.Coli
• EcoR11
• EcoR1
• EcoR111

16. Natural extra chromosomal circular DNA are choose which one is correct:

• Endonucleases
• Restriction enzyme
• Plasmids
• Phage virus

17. Which of the followings is not a vector?

• pSc 101
• pBR-322
• EcoR1
• Phage virus

18. The plasmids having resistant gene against tetracycline and ampicillin are choose which one is correct:

• pSc 101
• pBR-322
• EcoR1
• Phage virus

19. Which of the following phages is use as vector?

• Bacteriophage
• Lambda phage
• T phage
• All of the above

20. Pieces of DNA are jointed by choose which one is correct:

• Chimaeric DNA
• DA ligase
• Plasmids
• Endonucleases

21. Match recombinant DNA with one of the followings choose which one is correct:

• Chimaeric DNA
• DNA ligase
• Plasmids
• Endonucleases

22. Recombinant DNA are expressed in choose which one is correct:

• Man
• Virus
• Bacteria
• None of the above

23. The chemical that makes the bacterial membrane permeable is choose which one is correct:

• NaCl
• CaCl₂
• Na₂CO₃
• CaCO₃

24. The single standard nucleotide sequence that hybridize into certain piece is choose which one is correct:

• Clone
• Probe
• REFLP
• Endonuclease

25. Which of the followings form genomic library?

• Bacteria
• Bacteriophage
• Segment of DNA
• None of the above

26. PCR technique was developed by choose which one is correct:

• Kary Mullis
• Hamilton
• Sanger
• Maxam

27. Which of the followings is irrelevant for PCR?

• Primer DNA
• Recombinant DNA
• DNA polymerase
• Thermocycler

28. Match target DNA with one of the followings choose which one is correct:

• Primer DNA
• Recombinant DNA
• DNA polymerase
• Thermocycler

29. The enzyme extracted from Thermus aquaticus is choose which one is correct:

• Primer DNA
• Recombinant DNA
• DNA polymerase
• Thermocycler

30. The steps involved in DNA finger printing choose which one is correct:

• RFLP
• Probes
• PCR
• All of the above

31. The collection of different sized fragments is choose which one is correct:

• Probes
• RFLP
• PCR
• Gel

32. DNA analyzer is used for choose which one is correct:

• Forensic
• Diagnosis
• Evolution
• All of the above

33. The use of DNA analysis for convicting the criminal of rape is used in choose which one is correct:

• Diagnosis
• Forensic
• Evolution
• All of the above

34. Match Sanger’s method for generation of pieces of DNA with one of the

followings choose which one is correct:

• Deoxyribonucleotide
• Ribonucleoside
• Dideoxyribonucleoside
• DNA threads

35. Match Maxam  Gilbert method for generation of pieces of DNA with one of

the followings choose which one is correct:

• Deoxyribonucleotide
• Dexoyribonucleoside
• Dideoxyribonucleoside
• DMA threads

36. Pieces of DNA are separated on choose which one is correct:

• Agarol
• Agarose gel
• Agar
• All of the above

37. In gene sequencing the nucleotide strained with fluorescent dye are choose which one is correct:

• Initiation nucleotides
• Middle nucleotides
• Terminating nucleotides
• None of the above

38. In gene sequencing, the colour of the fluorescent bands are read by choose which one is correct:

• Computer
• Gel
• Detector
• Laser beam

39. The nucleotide sequence is stored by choose which one is correct:

• Computer
• Gel
• Detector
• Laser beam

40. The DNA sequence of which of the followings have been done?

• Chloroplast
• Mitochondria
• Yeast
• All of the above

41. Which of the followings is model plant whose gene sequencing has been completed?

• Yeast
• Pea
• Arabidopsis
• Plum

42. The gene sequence of which of the following chromosomes was completed in

1999 choose which one is correct:

• 12
• 18
• 21
• 22

43. The smallest human chromosomes is choose which one is correct:

• 10
• 22
• 23
• Y

44. Which of the followings helped scientist to pinpoint disease causing genes?

• PCR
• Recombinant DNA
• RFLP
• Probe

45. The number of base sequences in man is choose which one is correct:

• 2 billion
• 3 billion
• 4 billion
• 5 billion

46. The human genomic project will take how many volume of encyclopedia choose which one is correct:

• 100
• 200
• 300
• 400

47. Each piece of DNA should be choose which one is correct:

• 500 nucleotide
• 1000 nucleotide
• 3000 nucleotide
• 4000 nucleotide

48. The copy of DNA pieces in human genomic project is made by choose which one is correct:

• PCR
• Recombinant DNA
• RFLP
• Probe

49. The entire genome has been sequence by the company of choose which one is correct:

• J. Craig Venter
• Hamilton
• Sanger
• Maxam

50. Organisms with foreign genes are called choose which one is correct:

• Recombinant
• Hybrids
• Transgenic
• Modified

51. Transgenic bacteria are produced in large vats called choose which one is correct:

• Thermocycler
• Bioreactors
• Reactors
• Electrophoresis

52. Which of the following products is prepared by recombinant DNA technology?

• Hepatitis B vaccine
• Growth hormone
• Insulin
• All of the above

53. The degradation of pollutants with the help of recombinant bacteria is called choose which one is correct:

• Biofilters
• Biodegradation
• Bioabsorption
• None of the above

54. The bacteria used in industries for control of pollution are choose which one is correct:

• Bioabsorption
• Biodegradation
• Biofilters
• None of the above

55. The genes which cause self destruction of bacteria are choose which one is correct:

• Phage genes
• Suicide genes
• Recombinant genes
• Plasmid genes

56. Naturasweet is choose which one is correct:

• Sugar
• Starch
• Aspartame
• Glucose

57. The bacteria are used in mining industry for choose which one is correct:

• Bioabsorption
• Biodegradation
• Biofilters
• Bioleaching

58. The plant cells whose cell wall is removed are choose which one is correct:

• Naked cells
• Cullous
• Protoplast
• All of the above

59. In which of the followings plant pest and herb resistant genes are not used?

• Cotton
• Corm
• Yeast
• Potato

60. The acreage of transgenic plants in 1999 was choose which one is correct:

• 50 million
• 70 million
• 90 million
• 100 million

61. Green revolution was launched in choose which one is correct:

• 1960
• 1970
• 1990
• 2000

62. Scientists are working on which of followings for increasing yield choose which one is correct:

• Stomata
• RuBP carboxylase
• C4 cycle
• All of the above

63. Biodegradable plastic is obtained from choose which one is correct:

• Yeast
• Mouse eared cress
• Bacteria
• Bioreactos

64. The antibody which can deliver radio isotopes to tumor cells is obtained

from choose which one is correct:

• Yeast
• Weed
• Corn
• Wheat

65. The antibody which is used for the treatment of genital herps is obtained

from choose which one is correct:

• Yeast
• Soya bean
• Corn
• Wheat

66. Gene pharming is used for obtaining choose which one is correct:

• Milk
• Meat
• Antibodies
• Drugs

67. In gene pharming egg is fertilized choose which one is correct:

• In vitro
• In vivo
• In bioreactor
• None of the above

68. Antithrombin is produced in choose which one is correct:

• Sheep
• Goat
• Mice
• Bovine

69. The organisms which are used to produce human growth hormone in their

urine are choose which one is correct:

• Sheep
• Goat
• Mice
• Bovine

70. The production of genetically identical copies of the organisms by asexual

reproduction is called choose which one is correct:

• Recombinant DNA
• Cloning
• Gene pharming
• None of the above

71. The first animal cloned was choose which one is correct:

• Sheep
• Dolly
• Mice
• Bovine

72. In the cloning of mice the second nucleus is obtained from choose which one is correct:

• Uterus cells
• Cumulus cells
• Skin cells
• None of the above

73. The insertion of genetic material into human cells for the treatment of a

disorder is called

• Gene cloning
• Gene pharming
• Gene therapy
• Gene sequencing

74. The disease not treated by Ex vivo gene therapy is choose which one is correct:

• SCID
• Diabetes
• Hypercholesterolemia
• All of the above

75. The enzyme ADA is involved in maturation of choose which one is correct:

• RBC
• Platelets
• Lymphocyte
• Macrophages

76. The organism used as vector during treatment of SCID by gene therapy is choose which one is correct:

• Plasmids
• Phage
• Retroviruses
• None of the above

77. The genes for the treatment of SCID are introduced in choose which one is correct:

• Bone marrow
• Heart
• Liver
• None of the above

78. The hypercholesterolemia causes at young age choose which one is correct:

• Diabetes
• Blood pressure
• Heart attack
• All of the above

79. The genes for the treatment of hypercholesterolemia are introduced in choose which one is correct:

• Bone marrow
• Heart
• Liver
• None of the above

80. In which of these in vivo gene therapy is used?

• Cystic fibrosis
• Cancer
• Coronary angioplasty
• All of the above

81. In cystic fibrosis, the gene codes for trans membrane carrier of which ion is

missing choose which one is correct:

• Na
• K
• Cl
• Ca

82. The microscopic vesicles formed during gene therapy of cystic fibrosis are

called choose which one is correct:

• Ribosomes
• Lipososmes
• Endosomes
• Microsomes

83. The solution of Liposomes is sprayed on choose which one is correct:

• Skin
• Joints
• Nostrils
• Mouth

84. The gene therapy in cancer makes the patient tolerant for choose which one is correct:

• Radiotherapy
• Chemotherapy
• Endoscopies
• Gene therapy

85. These are used in angiosplasty choose which one is correct:

• Balloon
• Plasmids
• Gene therapy
• All of the above

86. Which of the following diseases is not treated by in vivo gene therapy?

• Haemophilia
• Diabetes
• Parkinson’s diseases
• All of the above

87. The plants cells can develop complete plants. So they are called choose which one is correct:

• Callous
• Totipotent
• Clone
• None of the above

88. Complete carrot plant was developed from tiny part of phloem by choose which one is correct:

• J. Craig Venter
• Hamilton
• Sanger
• F.C. Steward

89. F. C Steward provided the phloem cells choose which one is correct:

• Sugars
• Vitamins
• Coconut milk
• All of the above

90. An undifferentiated group of cells is called choose which one is correct:

• Callous
• Totipotent
• Clone
• None of the above

91. Callous can form choose which one is correct:

• Root
• Stem
• Shoot
• Complete plant

92. In micropropagation, the structure used is choose which one is correct:

• Stem
• Meristem
• Callous
• Totipotent

93. In micropropagation of meristem, the hormone used is choose which one is correct:

• Gibberellins
• Auxins
• Abscisic acid
• All of the above

94. The plants produced by micropropagation of meristem are called choose which one is correct:

• Callous
• Seedling
• Clonal
• Somaclonal

95. Which of the following is virus free?

• Callous
• Seedling
• Clonal plant
• Adult plant

96. The naked cells of plant are called choose which one is correct:

• Protoplasm
• Cytoplasm
• Protoplast
• Callous

97. The protoplast regenerates and produces choose which one is correct:

• Callous
• Seedling
• Clonal
• Clump

98. The clumps produce choose which one is correct:

• Callous
• Somatic embryo
• Clonal plant
• Adult plant

99. Somatic embryo are encapsulated in gel and are called choose which one is correct:

• Seeds
• Artificial seed
• Clonal plant
• Clump

100. Millions of artificial seed are produced in large tanks called choose which one is correct:

• Thermocycler
• Biopole
• Bioreactors
• None of the above

101. The artificial seeds are produced of which of the following plants?

• Corn
• Tomato
• Mango
• Roses

102. The plants produced by micropropagation from single cell are called choose which one is correct:

• Callous
• Seedling
• Clonal
• Somaclonal

103. Anther is cultured in a medium containing choose which one is correct:

• Sugars
• Vitamins
• Coconut milk
• All of the above