12th Class Biology Mcqs with Answers Chapter 23 | 2nd year Biology Mcqs With Answers

Chapter 23

Biotechnology

Encircle the most appropriate answer among the following options

1. Mendel’s work was rediscovered in choose which one is correct:

  • 1868
  • 1882
  • 1900
  • 1950

2. The work of biotechnology is choose which one is correct:

  • Insulin
  • Drugs
  • Pollution
  • All of the above

3. Production of identical copying of gene is choose which one is correct:

  • Replication
  • Cloning
  • Reproduction
  • Multiplication

4. Which of the following techniques produces large number of copies of genes?

  • PCR
  • Recombinant DNA technology
  • RFLP
  • None of the above

5. The technique used to produce copies of genes in laboratory is choose which one is correct:

  • PCR
  • Recombinant DNA technology
  • RFLOP
  • None of the above

6. Which of the followings is used to transfer genes?

  • Molecular scissors
  • Molecular vector
  • Expression system
  • None of the above

7. The system used for the formation of specific product is choose which one is correct:

  • Molecular scissors
  • Molecular vector
  • Expression system
  • Gene of interest

8. Genes are cut by choose which one is correct:

  • Gene of interest
  • Expression system
  • Molecular vector
  • Endonucleases

9. The genes which are synthesized from mRNA by reverse transcriptase are

called choose which one is correct:

  • Endonucleases
  • Plasmids
  • Coda
  • None of the above

10. Restriction enzymes are present in choose which one is correct:

  • Virus
  • Bacteria
  • Plasmids
  • All of the above

11. The first restriction enzyme was isolated by choose which one is correct:

  • Kary Mullis
  • Hamiton
  • Sanger
  • Maxam

12. Palindromic sequences are present in choose which one is correct:

  • Single phase
  • Repeated form
  • Reverse order
  • Similar order

13. Number of restriction enzymes discovered so far are choose which one is correct:

  • 200
  • 300
  • 400
  • 500

14. The number of frequently used restriction enzymes are choose which one is correct:

  • 10
  • 20
  • 30
  • 40

15. The commonly used restriction enzyme is choose which one is correct:

  • E.Coli
  • EcoR11
  • EcoR1
  • EcoR111

16. Natural extra chromosomal circular DNA are choose which one is correct:

  • Endonucleases
  • Restriction enzyme
  • Plasmids
  • Phage virus

17. Which of the followings is not a vector?

  • pSc 101
  • pBR-322
  • EcoR1
  • Phage virus

18. The plasmids having resistant gene against tetracycline and ampicillin are choose which one is correct:

  • pSc 101
  • pBR-322
  • EcoR1
  • Phage virus

19. Which of the following phages is use as vector?

  • Bacteriophage
  • Lambda phage
  • T phage
  • All of the above

20. Pieces of DNA are jointed by choose which one is correct:

  • Chimaeric DNA
  • DA ligase
  • Plasmids
  • Endonucleases

21. Match recombinant DNA with one of the followings choose which one is correct:

  • Chimaeric DNA
  • DNA ligase
  • Plasmids
  • Endonucleases

22. Recombinant DNA are expressed in choose which one is correct:

  • Man
  • Virus
  • Bacteria
  • None of the above

23. The chemical that makes the bacterial membrane permeable is choose which one is correct:

  • NaCl
  • CaCl2
  • Na2CO3
  • CaCO3

24. The single standard nucleotide sequence that hybridize into certain piece is choose which one is correct:

  • Clone
  • Probe
  • REFLP
  • Endonuclease

25. Which of the followings form genomic library?

  • Bacteria
  • Bacteriophage
  • Segment of DNA
  • None of the above

26. PCR technique was developed by choose which one is correct:

  • Kary Mullis
  • Hamilton
  • Sanger
  • Maxam

27. Which of the followings is irrelevant for PCR?

  • Primer DNA
  • Recombinant DNA
  • DNA polymerase
  • Thermocycler

28. Match target DNA with one of the followings choose which one is correct:

  • Primer DNA
  • Recombinant DNA
  • DNA polymerase
  • Thermocycler

29. The enzyme extracted from Thermus aquaticus is choose which one is correct:

  • Primer DNA
  • Recombinant DNA
  • DNA polymerase
  • Thermocycler

30. The steps involved in DNA finger printing choose which one is correct:

  • RFLP
  • Probes
  • PCR
  • All of the above

31. The collection of different sized fragments is choose which one is correct:

  • Probes
  • RFLP
  • PCR
  • Gel

32. DNA analyzer is used for choose which one is correct:

  • Forensic
  • Diagnosis
  • Evolution
  • All of the above

33. The use of DNA analysis for convicting the criminal of rape is used in choose which one is correct:

  • Diagnosis
  • Forensic
  • Evolution
  • All of the above

34. Match Sanger’s method for generation of pieces of DNA with one of the

followings choose which one is correct:

  • Deoxyribonucleotide
  • Ribonucleoside
  • Dideoxyribonucleoside
  • DNA threads

35. Match Maxam  Gilbert method for generation of pieces of DNA with one of

the followings choose which one is correct:

  • Deoxyribonucleotide
  • Dexoyribonucleoside
  • Dideoxyribonucleoside
  • DMA threads

36. Pieces of DNA are separated on choose which one is correct:

  • Agarol
  • Agarose gel
  • Agar
  • All of the above

37. In gene sequencing the nucleotide strained with fluorescent dye are choose which one is correct:

  • Initiation nucleotides
  • Middle nucleotides
  • Terminating nucleotides
  • None of the above

38. In gene sequencing, the colour of the fluorescent bands are read by choose which one is correct:

  • Computer
  • Gel
  • Detector
  • Laser beam

39. The nucleotide sequence is stored by choose which one is correct:

  • Computer
  • Gel
  • Detector
  • Laser beam

40. The DNA sequence of which of the followings have been done?

  • Chloroplast
  • Mitochondria
  • Yeast
  • All of the above

41. Which of the followings is model plant whose gene sequencing has been completed?

  • Yeast
  • Pea
  • Arabidopsis
  • Plum

42. The gene sequence of which of the following chromosomes was completed in

1999 choose which one is correct:

  • 12
  • 18
  • 21
  • 22

43. The smallest human chromosomes is choose which one is correct:

  • 10
  • 22
  • 23
  • Y

44. Which of the followings helped scientist to pinpoint disease causing genes?

  • PCR
  • Recombinant DNA
  • RFLP
  • Probe

45. The number of base sequences in man is choose which one is correct:

  • 2 billion
  • 3 billion
  • 4 billion
  • 5 billion

46. The human genomic project will take how many volume of encyclopedia choose which one is correct:

  • 100
  • 200
  • 300
  • 400

47. Each piece of DNA should be choose which one is correct:

  • 500 nucleotide
  • 1000 nucleotide
  • 3000 nucleotide
  • 4000 nucleotide

48. The copy of DNA pieces in human genomic project is made by choose which one is correct:

  • PCR
  • Recombinant DNA
  • RFLP
  • Probe

49. The entire genome has been sequence by the company of choose which one is correct:

  • J. Craig Venter
  • Hamilton
  • Sanger
  • Maxam

50. Organisms with foreign genes are called choose which one is correct:

  • Recombinant
  • Hybrids
  • Transgenic
  • Modified

51. Transgenic bacteria are produced in large vats called choose which one is correct:

  • Thermocycler
  • Bioreactors
  • Reactors
  • Electrophoresis

52. Which of the following products is prepared by recombinant DNA technology?

  • Hepatitis B vaccine
  • Growth hormone
  • Insulin
  • All of the above

53. The degradation of pollutants with the help of recombinant bacteria is called choose which one is correct:

  • Biofilters
  • Biodegradation
  • Bioabsorption
  • None of the above

54. The bacteria used in industries for control of pollution are choose which one is correct:

  • Bioabsorption
  • Biodegradation
  • Biofilters
  • None of the above

55. The genes which cause self destruction of bacteria are choose which one is correct:

  • Phage genes
  • Suicide genes
  • Recombinant genes
  • Plasmid genes

56. Naturasweet is choose which one is correct:

  • Sugar
  • Starch
  • Aspartame
  • Glucose

57. The bacteria are used in mining industry for choose which one is correct:

  • Bioabsorption
  • Biodegradation
  • Biofilters
  • Bioleaching

58. The plant cells whose cell wall is removed are choose which one is correct:

  • Naked cells
  • Cullous
  • Protoplast
  • All of the above

59. In which of the followings plant pest and herb resistant genes are not used?

  • Cotton
  • Corm
  • Yeast
  • Potato

60. The acreage of transgenic plants in 1999 was choose which one is correct:

  • 50 million
  • 70 million
  • 90 million
  • 100 million

61. Green revolution was launched in choose which one is correct:

  • 1960
  • 1970
  • 1990
  • 2000

62. Scientists are working on which of followings for increasing yield choose which one is correct:

  • Stomata
  • RuBP carboxylase
  • C4 cycle
  • All of the above

63. Biodegradable plastic is obtained from choose which one is correct:

  • Yeast
  • Mouse eared cress
  • Bacteria
  • Bioreactos

64. The antibody which can deliver radio isotopes to tumor cells is obtained

from choose which one is correct:

  • Yeast
  • Weed
  • Corn
  • Wheat

65. The antibody which is used for the treatment of genital herps is obtained

from choose which one is correct:

  • Yeast
  • Soya bean
  • Corn
  • Wheat

66. Gene pharming is used for obtaining choose which one is correct:

  • Milk
  • Meat
  • Antibodies
  • Drugs

67. In gene pharming egg is fertilized choose which one is correct:

  • In vitro
  • In vivo
  • In bioreactor
  • None of the above

68. Antithrombin is produced in choose which one is correct:

  • Sheep
  • Goat
  • Mice
  • Bovine

69. The organisms which are used to produce human growth hormone in their

urine are choose which one is correct:

  • Sheep
  • Goat
  • Mice
  • Bovine

70. The production of genetically identical copies of the organisms by asexual

reproduction is called choose which one is correct:

  • Recombinant DNA
  • Cloning
  • Gene pharming
  • None of the above

71. The first animal cloned was choose which one is correct:

  • Sheep
  • Dolly
  • Mice
  • Bovine

72. In the cloning of mice the second nucleus is obtained from choose which one is correct:

  • Uterus cells
  • Cumulus cells
  • Skin cells
  • None of the above

73. The insertion of genetic material into human cells for the treatment of a

disorder is called

  • Gene cloning
  • Gene pharming
  • Gene therapy
  • Gene sequencing

74. The disease not treated by Ex vivo gene therapy is choose which one is correct:

  • SCID
  • Diabetes
  • Hypercholesterolemia
  • All of the above

75. The enzyme ADA is involved in maturation of choose which one is correct:

  • RBC
  • Platelets
  • Lymphocyte
  • Macrophages

76. The organism used as vector during treatment of SCID by gene therapy is choose which one is correct:

  • Plasmids
  • Phage
  • Retroviruses
  • None of the above

77. The genes for the treatment of SCID are introduced in choose which one is correct:

  • Bone marrow
  • Heart
  • Liver
  • None of the above

78. The hypercholesterolemia causes at young age choose which one is correct:

  • Diabetes
  • Blood pressure
  • Heart attack
  • All of the above

79. The genes for the treatment of hypercholesterolemia are introduced in choose which one is correct:

  • Bone marrow
  • Heart
  • Liver
  • None of the above

80. In which of these in vivo gene therapy is used?

  • Cystic fibrosis
  • Cancer
  • Coronary angioplasty
  • All of the above

81. In cystic fibrosis, the gene codes for trans membrane carrier of which ion is

missing choose which one is correct:

  • Na
  • K
  • Cl
  • Ca

82. The microscopic vesicles formed during gene therapy of cystic fibrosis are

called choose which one is correct:

  • Ribosomes
  • Lipososmes
  • Endosomes
  • Microsomes

83. The solution of Liposomes is sprayed on choose which one is correct:

  • Skin
  • Joints
  • Nostrils
  • Mouth

84. The gene therapy in cancer makes the patient tolerant for choose which one is correct:

  • Radiotherapy
  • Chemotherapy
  • Endoscopies
  • Gene therapy

85. These are used in angiosplasty choose which one is correct:

  • Balloon
  • Plasmids
  • Gene therapy
  • All of the above

86. Which of the following diseases is not treated by in vivo gene therapy?

  • Haemophilia
  • Diabetes
  • Parkinson’s diseases
  • All of the above

87. The plants cells can develop complete plants. So they are called choose which one is correct:

  • Callous
  • Totipotent
  • Clone
  • None of the above

88. Complete carrot plant was developed from tiny part of phloem by choose which one is correct:

  • J. Craig Venter
  • Hamilton
  • Sanger
  • F.C. Steward

89. F. C Steward provided the phloem cells choose which one is correct:

  • Sugars
  • Vitamins
  • Coconut milk
  • All of the above

90. An undifferentiated group of cells is called choose which one is correct:

  • Callous
  • Totipotent
  • Clone
  • None of the above

91. Callous can form choose which one is correct:

  • Root
  • Stem
  • Shoot
  • Complete plant

92. In micropropagation, the structure used is choose which one is correct:

  • Stem
  • Meristem
  • Callous
  • Totipotent

93. In micropropagation of meristem, the hormone used is choose which one is correct:

  • Gibberellins
  • Auxins
  • Abscisic acid
  • All of the above

94. The plants produced by micropropagation of meristem are called choose which one is correct:

  • Callous
  • Seedling
  • Clonal
  • Somaclonal

95. Which of the following is virus free?

  • Callous
  • Seedling
  • Clonal plant
  • Adult plant

96. The naked cells of plant are called choose which one is correct:

  • Protoplasm
  • Cytoplasm
  • Protoplast
  • Callous

97. The protoplast regenerates and produces choose which one is correct:

  • Callous
  • Seedling
  • Clonal
  • Clump

98. The clumps produce choose which one is correct:

  • Callous
  • Somatic embryo
  • Clonal plant
  • Adult plant

99. Somatic embryo are encapsulated in gel and are called choose which one is correct:

  • Seeds
  • Artificial seed
  • Clonal plant
  • Clump

100. Millions of artificial seed are produced in large tanks called choose which one is correct:

  • Thermocycler
  • Biopole
  • Bioreactors
  • None of the above

101. The artificial seeds are produced of which of the following plants?

  • Corn
  • Tomato
  • Mango
  • Roses

102. The plants produced by micropropagation from single cell are called choose which one is correct:

  • Callous
  • Seedling
  • Clonal
  • Somaclonal

103. Anther is cultured in a medium containing choose which one is correct:

  • Sugars
  • Vitamins
  • Coconut milk
  • All of the above